Methods The functions of GJIC in bladder smooth muscle were detected by fluorescence recovery after photobleaching ( FRAP). 方法利用荧光光漂白恢复(FRAP)技术检测膀胱平滑肌细胞GJIC功能。
A variety of photonics technologies in molecular biology, such as fluorescence recovery after photobleaching ( FRAP), fluorescence resonance energy transfer ( FRET), fluorescence correlation spectroscopy ( FCS), surface plasmon resonance ( SPR), and so on were introduced. 综述了如荧光漂白恢复(FRAP)、荧光共振能量转移(FRET)、荧光相关光谱(FCS)、表面质膜共振(SPR)等光学方法在生物分子研究中的应用。
Fluorescence recovery after photobleaching ( FRAP) was applied to analyze the function of GJIC in stromal and epithelial cells with and without EMs. Furthermore, GJIC status in epithelial cells regulated by different stromal cells was evaluated in co-culture system in vitro. 采用荧光漂白后恢复技术分析三组内膜间质细胞和上皮细胞的GJIC功能,并探讨共培养体系中EMs内膜间质细胞对不同上皮细胞GJIC功能的调节作用。